IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 744
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
2,4 Dimethylamine
9.728
0.05
0.76
Commercial Formulation Clean Crop
Guinea pig
Dermatomed to 0.3mm
Back
0.64
Flow-through
HBSS, HEPES buffer, BSA & gentamycin sulphate
37
Incubator
24
48
2
Radiolabelled(14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
100.3
1.17
2.04
9.4
16.9
7.5
2.52
3.6
1.88
4
NOTES
Hairless 9 month old Guinea-Pigs were used. % Penetrated was calculated by adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption
REFERENCE
In Vitro Dermal Absorption Of Two Commercial Formulations Of 2,4-Dichlorophenoxyacetic Acid Dimethylamine (2,4-D Amine) in Rat, Guinea Pig and Human Skin (1997) Toxicol. Vitro, 11 :251-262