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In Vitro Study No: 745

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2,4 Dimethylamine9.7280.050.76Commercial Formulation Wilbur-EllisGuinea pigDermatomed to 0.3mmBack0.64Flow-throughHBSS, HEPES buffer, BSA & gentamycin sulphate37Incubator24482Radiolabelled(14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
95.83.74 1.256.9 12.8 5.93.723.60.82 4 

NOTES

Hairless 9 month old Guinea-Pigs were used. % Penetrated was calculated by adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption

REFERENCE

In Vitro Dermal Absorption Of Two Commercial Formulations Of 2,4-Dichlorophenoxyacetic Acid Dimethylamine (2,4-D Amine) in Rat, Guinea Pig and Human Skin (1997) Toxicol. Vitro, 11 :251-262