IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 747
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
2,4 Dimethylamine
9.728
0.05
0.756
Commercial Formulation Wilbur-Ellis
Human
Dermatomed to 0.3mm
Abdomen
0.64
Flow-through
HBSS, HEPES buffer, BSA & gentamycin sulphate
37
Incubator
24
48
2
Radiolabelled(14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
94.6
3.08
0.23
10.9
21.8
10.9
1.31
7.9
19.3
4
NOTES
The skin cam from the abdomen of a 38 year old Caucasian after surgery. % Penetrated was calculated by adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption
REFERENCE
In Vitro Dermal Absorption Of Two Commercial Formulations Of 2,4-Dichlorophenoxyacetic Acid Dimethylamine (2,4-D Amine) in Rat, Guinea Pig and Human Skin (1997) Toxicol. Vitro, 11 :251-262