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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 752

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Paraoxon

1.28

0.01

0.04

Ethanol

Pig

Dermatomed to 0.5mm

Dorsal

0.32

Flow-through

Kreb's-Ringer bicarbonate buffer & BSA

Receptor Fluid

Radiolabelled (14C) + HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								9.38	2.9				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

9.38

2.9

NOTES

Weanling Pigs were used. The HPLC was used to determine the % of absorbed chemical that existed as metabolites

REFERENCE

Percutaneous Absorption Of Parathion And Its Metabolites, Paraoxon and P-Nitrophenol, Administered Alone Or In Combination:In Vitro Flow-through Diffusion Studies (1994) Pestic. Biochem. Physiol., 48 :56-62