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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 754

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
4-Nitrophenol0.1280.010.004EthanolPigDermatomed to 0.5mmDorsal0.32Flow-throughKreb's-Ringer bicarbonate buffer & BSA37Receptor Fluid882Radiolabelled (14C) + HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        11.212.28   4 

NOTES

Weanling Pigs were used. The HPLC was used to determine the % of absorbed chemical that existed as metabolites

REFERENCE

Percutaneous Absorption Of Parathion And Its Metabolites, Paraoxon and P-Nitrophenol, Administered Alone Or In Combination:In Vitro Flow-through Diffusion Studies (1994) Pestic. Biochem. Physiol., 48 :56-62