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In Vitro Study No: 77

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Caffeine   PetrolatumTestskin TM3microm pore size polycarbonate membraneN/A0.8StaticLDE Assay Medium22Water Bath24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.00113 

NOTES

0.2microCi and 2microg unlabelled caffeine was added/0.15g petrolatum. 0.15g was added.. Samples were taken at hourly intervals and Kp calculated from s-state portion of curves % applied dose/h/cm2

REFERENCE

The Living Skin Equivalent As A Model In Vitro For Ranking The Toxic Potential Of Dermal Irritants (1992) Toxicol. Vitro, 6 :303-315