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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 772

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Paraoxon

0.064

0.01

0.002

Ethanol + 4-Nitrophenol

Pig

Dermatomed to 0.5mm

Dorsal

0.32

Flow-through

Kreb's-Ringer bicarbonate buffer & BSA

Receptor Fluid

Radiolabelled (14C) + HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								14.75	32.2				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

14.75

32.2

NOTES

Weanling Pigs were used. The HPLC was used to differentiate between the absorbed Paraoxon and 4-Nitrophenol. The Paraoxon and 4-Nitrophenol were administered as a 1:1 mixture

REFERENCE

Percutaneous Absorption Of Parathion And Its Metabolites, Paraoxon and P-Nitrophenol, Administered Alone Or In Combination:In Vitro Flow-through Diffusion Studies (1994) Pestic. Biochem. Physiol., 48 :56-62