EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

 University of Newcastle homepage

In Vitro Study No: 78

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid 1 WaterTestskin TM3microm pore size polycarbonate membraneN/A0.5StaticLDE Assay Medium32Water Bath24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.0863 

NOTES

Did not say how much Benzoic Acid was added/ml water. Samples were taken at hourly intervals and Kp calculated from s-state portion of curves % applied dose/h/cm2

REFERENCE

The Living Skin Equivalent As A Model In Vitro For Ranking The Toxic Potential Of Dermal Irritants (1992) Toxicol. Vitro, 6 :303-315