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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 783

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Carbaryl

2.56

0.01

0.04

Ethanol

Pig

Dermatomed tp 0.5mm

Dorsal

0.64

Flow-through

Krebs-Ringer Bicarbonate buffer, 4.5% BSA & glucose

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								9.49	2.42				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

9.49

2.42

NOTES

Female Weanling Yorkshire Pigs were used.Every day for 10 days before the pig was sacrificed a dose of 1ml of 100% ethanol was added to the dosing site. The skin was allowed to equilibrate for 1 hour in the flow-through cells before the dose solution was added. The details of the area of skin exposed in the flow-though cell were obtained from ref No 44, and this was used to calculate the % absorption as the results were simply given as micrgrams/8h.

REFERENCE

Enhancing Or Blocking Effect Of Fenvalerate On The Subsequent Percutansous Abosorption Of Pesticides In Vitro (1995) Pestic. Biochem. Physiol., 51 :214-219