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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 80

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzoic Acid

Petrolatum

Testskin TM

3microm pore size polycarbonate membrane

N/A

0.8

Static

LDE Assay Medium

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.0067	3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0067

NOTES

Did not say how much Benzoic Acid was added. They used 0.15g Petrolatum.Samples were taken at hourly intervals and Kp calculated from s-state portion of curves % applied dose/h/cm2

REFERENCE

The Living Skin Equivalent As A Model In Vitro For Ranking The Toxic Potential Of Dermal Irritants (1992) Toxicol. Vitro, 6 :303-315