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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 802

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Arsenic

See Notes

0.0006

Soil

Human

Dermatomed to 0.5mm

Not Given

Flow-through

PBS

Not Given

Radiolabelled (73As)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
99.9	27.5		0.25	0.33		0.76		0.43	0.54				9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

99.9

27.5

0.25

0.33

0.76

0.43

0.54

NOTES

Human cadaver skin was used. The soil sample came form Yolo County, California (26% sand, 26% clay, 48% silt 0.9% organic) 40mg of soil was used. The Potential Absorption was calculated by adding together the amount found in the receptor fluid to the amount found in the skin. The actual amount absorbed in mg was extremely small - 2.6E-6

REFERENCE

In Vivo And In Vitro Percutaneous Absorption And Skin Decontamination Of Arsenic From Water And Soil (1993) Fundam. Appl. Toxicol., 20 :336-340