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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 804

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Methyl nicotinate

Infinite

1368.5

Transcutol

Rat

Full Thickness Skin

Abdomen

2.54

Static

0.9~% sodium chloride

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										2820		0.00325	5	1.25

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2820

0.00325

1.25

NOTES

Transcutol is diethylene glycol monomethyl ether. 2g of the vehicle was applied, which was saturated with methyl nicotinate. 8-week old hairless rats were used. Flux was calculated from Kp multiplied by the solubility in mg/g. For methyl nicotinate in Transcutol this was 869mg/g. The Kp was calculated using a Mathematica programme.

REFERENCE

Percutaneous Absorption Of Methyl Nicotinate (1995) Int. J. Pharm., 121 :