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In Vitro Study No: 807

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Methyl nicotinate Infinite1368.5Propylene glycol dipelargonateRatFull Thickness SkinAbdomen2.54Static0.9~% sodium chloride30Skin 02HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          3560 0.0066350.78

NOTES

2g of the vehicle was applied, which was saturated with methyl nicotinate. 8-week old hairless rats were used. Flux was calculated from Kp multiplied by the solubility in mg/g. For methyl nicotinate in DPPG this was 537mg/g. The Kp was calculated using a Mathematica programme.

REFERENCE

Percutaneous Absorption Of Methyl Nicotinate (1995) Int. J. Pharm., 121 :