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In Vitro Study No: 809

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Urea Infinite0.0004PetrolatumRatFull Thickness SkinBack1.13StaticSaline & Thimerosol32Water Bath  2Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        7.2   1.6E-054 

NOTES

Female Osborne Mendel Rats aged 3-6 months old were used. 30mg of petrolatum was added in total. The concentration of Urea in the Petrolatum was 15ng/mg

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies I. Comparison With In Vivo Studies (1982) Toxicol. Appl. Pharmacol., 62 :474-480