IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 809
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Urea
Infinite
0.0004
Petrolatum
Rat
Full Thickness Skin
Back
1.13
Static
Saline & Thimerosol
32
Water Bath
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
7.2
1.6E-05
4
NOTES
Female Osborne Mendel Rats aged 3-6 months old were used. 30mg of petrolatum was added in total. The concentration of Urea in the Petrolatum was 15ng/mg
REFERENCE
Methods For In Vitro Percutaneous Absorption Studies I. Comparison With In Vivo Studies (1982) Toxicol. Appl. Pharmacol., 62 :474-480