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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 810

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Acetylsalicylic Acid

Infinite

0.00664

Petrolatum

Rat

Full Thickness Skin

Back

1.13

Static

Saline & Thimerosol

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								29	6.2			6.5E-05	4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

6.2

6.5E-05

NOTES

Female Osborne Mendel Rats aged 3-6 months old were used. 30mg of petrolatum was added in total. The concentration of Acetylsalicylic acid in the Petrolatum was 250ng/mg

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies I. Comparison With In Vivo Studies (1982) Toxicol. Appl. Pharmacol., 62 :474-480