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In Vitro Study No: 816

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
MDA See Notes EthanolRatFull Thickness SkinDorsal0.32Flow-throughHHBSS & 0.5% gentamycin32Skin72722Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
   5.657.6 63.7 6.12   122

NOTES

Male Fischer 344 Rats were used. The dose applied was between 5 and 10 mcl. Dosing levels ranged from 20.1-39.4 mcg/cm2. % Penetrated has been calculated by adding together the amount found in the receptor fluid and the amount found in the skin.

REFERENCE

Percutaneous Absorption Of 4,4'Methylene-Bis-(2-Chloroaniline) And 4,4'Methylenedianiline Through Rat And Human Skin In Vitro (1993) Toxicol. Vitro, 7 :141-148