IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 816
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
MDA
See Notes
Ethanol
Rat
Full Thickness Skin
Dorsal
0.32
Flow-through
HHBSS & 0.5% gentamycin
32
Skin
72
72
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
5.6
57.6
63.7
6.1
2
12
2
NOTES
Male Fischer 344 Rats were used. The dose applied was between 5 and 10 mcl. Dosing levels ranged from 20.1-39.4 mcg/cm2. % Penetrated has been calculated by adding together the amount found in the receptor fluid and the amount found in the skin.
REFERENCE
Percutaneous Absorption Of 4,4'Methylene-Bis-(2-Chloroaniline) And 4,4'Methylenedianiline Through Rat And Human Skin In Vitro (1993) Toxicol. Vitro, 7 :141-148