EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 82

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Beta-Estradiol

Petrolatum

Testskin TM

3microm pore size polycarbonate membrane

N/A

0.8

Static

LDE Assay Medium

Water Bath

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00093	3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00093

NOTES

2microCi and 2microg of Beta-Estradiol was added/0.15g Petrolatum. 0.15g was added. Samples were taken at hourly intervals and Kp calculated from s-state portion of curves % applied dose/h/cm2

REFERENCE

The Living Skin Equivalent As A Model In Vitro For Ranking The Toxic Potential Of Dermal Irritants (1992) Toxicol. Vitro, 6 :303-315