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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 823

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Thiourea

0.01

Acetone

Human

Full Thickness Skin

Abdomen

Static

NaCl, sodium pyrithione, , KH2PO4, K2HPO4

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								3.4					52

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

3.4

NOTES

Human cadavar skin was used. Following mounting each piece of skin was allowed to stand for several hours before biginning the experiment. The dose was between 4 and 40 mcrg/cm2. Depending on the Specific Activity of the compound used.They also used cells with a 2.5cm2 Area for diffusion. In these cells they used a dose of 0.025ml. The value given as % Absorption is the median value.

REFERENCE

Percutaneous Absorption. On The Relevance Of In Vitro Data (1975) J. Investig. Dermatol., 64 :190-195