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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 83

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Nicotine

Not Given

0.05M Phosphate Buffer

Human

Dermatomed to 0.408mm

Abdomen/Breast

1.77

Static

0.05M Phosphate buffer

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										206		0.0103	9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

206

0.0103

NOTES

The cells were closed with a cap to prevent evaporation. The skin was taken from 3 caucasian female donors, 3 samples of skin were used from each donor. Lag time was extremely short

REFERENCE

Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68