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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 834

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Cypermethrin

28.4

0.0005

0.00559

oil based spray

Human

Isolated Epidermis

Abdomen

2.54

Static

50% aq. Ethanol

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0.5	0.35				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.5

0.35

NOTES

Human cadaver skin was used. Human epidermal sheets were produced by immersing the skin in water at 60C for 45s and gently peeling away the epidermis. Results were given +/- SEM. I have converted this to SD for the purpose of this database. Eperiments were also conducted with Full thickness human skin, and with different receptor fluids such as saline. 6% VOLPO 20 in saline, PBS, 20% fetal calf serum in saline, 6% PEG-400 in saline. But no absorption was detectable in these studies.

REFERENCE

Comparison Of The In Vivo And In Vitro Percutaneous Absorption Of A Lipophilic Molecule (Cypermethrin, a Pyrethroid Insecticide) (1987) J. Investig. Dermatol., 89 :142-146