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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 837

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Cypermethrin

28.4

0.0005

0.00559

oil based spray

Rat

Isolated Epidermis

Dorso-lumbar region

2.54

Static

20% fetal calf serum in saline

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								2.6	1				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2.6

NOTES

Alpk/AP strain Wistar derived rats were used. Rat epidermal membranes were prepared by soaking in 2M NaBr solution before gently peeling away the epidermis. Results were given +/- SEM. I have converted this to SD for the purpose of this database.

REFERENCE

Comparison Of The In Vivo And In Vitro Percutaneous Absorption Of A Lipophilic Molecule (Cypermethrin, a Pyrethroid Insecticide) (1987) J. Investig. Dermatol., 89 :142-146