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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 84

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Salicylic acid

Not Given

0.05M Phosphate Buffer

Human

Dermatomed to 0.408mm

Abdomen/Breast

1.77

Static

0.05M Phosphate buffer

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										26.2		0.0131	9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

26.2

0.0131

NOTES

The Log Kp was given in the paper as -1.58 which gives a Kp of 2.62E-2. I think that this is an arithmetic error so I have changed it for the database. The cells were closed with a cap to prevent evaporation. The solution was adjusted to pH 2.5. The skin was taken from 3 caucasian female donors, 3 samples of skin were used from each donor. Lag time was in the range of 1-2 hours.

REFERENCE

Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68