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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 841

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Water

1000

0.318

None (Neat)

Mouse

Full Thickness Skin

Not Given

3.14

Flow-through

PBS

Skin

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00295	8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00295

NOTES

Hairless Mice were used. The cells were covered to prevent evaporation. The permeability coefficient has been calculated for between 3 and 20h post-application. The flux increased after 24 hours implying that the barrier had broken down due to 24h of hydration

REFERENCE

In Vitro Percutaneous Penetration: Evaluation Of The Utility Of Hairless Mouse Skin (1989) J. Investig. Dermatol., 93 :87-91