IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 85
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Testosterone
0.1
Not Given
0.05M Phosphate Buffer/20% Ethanol
Human
Dermatomed to 0.408mm
Abdomen/Breast
1.77
Static
0.05M Phosphate buffer
32
Skin
28
28
1
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.501
0.005012
9
NOTES
The cells were closed with a cap to prevent evaporation. The skin was taken from 3 caucasian female donors, 3 samples of skin were used from each donor.Lag time was in the range 3-5 hours
REFERENCE
Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68