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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 858

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzyl Acetate

5030

0.005

8.28

50% aq. Ethanol

Rat

Full Thickness Skin

Dorsal

0.32

Flow-through

0.9% Saline

Skin

Radiolbelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
						65.3		52.4	1.1	290			16

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

65.3

52.4

1.1

290

NOTES

Male Fischer 344 rats were used. The cells were occluded with Parafilm. % Penetrated was calculated by adding together the amount recovered in the receptor fluid to the amount recovered in the skin after washing.

REFERENCE

Percutaneous Absorption of Benzyl Acetate Through Rat Skin In Vitro.1. Validation Of An In Vitro Model Against In Vivo Data (1990) Food Chem.Toxicol., 28 :443-447