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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 87

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Salicylic acid

Not Given

0.05M Phosphate Buffer

Pig

Dermatomed to 0.765mm

Back

1.77

Static

0.05M Phosphate buffer

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										25.7		0.01285	9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

25.7

0.01285

NOTES

The Log Kp was given in the paper as -1.59 which gives a Kp of 2.57E-2. I think that this is an arithmetic error so I have changed it for the database.The cells were closed with a cap to prevent evaporation. The skins were from animals of weight 20kg. The solution was adjusted to pH 2.5 (2 months old - corresponding to an adolescent minipig). Lag time was in the range 1-2 hours

REFERENCE

Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68