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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 88

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Testosterone

0.1

Not Given

0.05M Phosphate Buffer/20% Ethanol

Pig

Dermatomed to 0.765mm

Back

1.77

Static

0.05M Phosphate buffer

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.792		0.00792	9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.792

0.00792

NOTES

The cells were closed with a cap to prevent evaporation. The skins were from animals of weight 20kg (2 months old - corresponding to an adolescent minipig). Lag time was in the range 3-5 hours

REFERENCE

Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68