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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 885

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Trichloroethylene

1470

0.005

9.36

None (Neat)

Rat

Full Thickness Skin

Flank

0.785

Static

Tyrodes Solution & BSA

Not Given

Radilabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0.5	0.2				5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.5

0.2

NOTES

Male Sprague-Dawley Rats were used. In the paper they state the loading as 9.36mcrgrams/cm2 but I think that they have made an error in their calculations so I have stated it as 9.36mg/cm2

REFERENCE

The Role Of Testosterone In Trichloroethylene Penetration In Vitro (1991) Environ. Res, 54 :82-92