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In Vitro Study No: 89

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Nicotine20Not Given 0.05M Phosphate BufferMinipigDermatomed to 0.765mmBack1.77Static0.05M Phosphate buffer32Skin28281HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          174 0.00879 

NOTES

The cells were closed with a cap to prevent evaporation. The skins were from animals 1.5 months old of weight 3kg. Lag time was extremely short.

REFERENCE

Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68