IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 89
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Nicotine
20
Not Given
0.05M Phosphate Buffer
Minipig
Dermatomed to 0.765mm
Back
1.77
Static
0.05M Phosphate buffer
32
Skin
28
28
1
HPLC
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
174
0.0087
9
NOTES
The cells were closed with a cap to prevent evaporation. The skins were from animals 1.5 months old of weight 3kg. Lag time was extremely short.
REFERENCE
Evaluation Of Gottingen Minipig Skin For Transdermal In Vitro Permeation Studies (2000) Eur. J. Pharm. Sci., 11 :59-68