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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 967

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Diethyl Malonate

0.01

Acetone

Pig

Dermatomed to 1mm

Back

0.8

Flow-through

RPMI media 1640 & gentamycin sulphate

Water Bath

Radiolabelled (14C) & TLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
28				9.5				17					9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

9.5

NOTES

The donor cell was occluded with a glass stopper to retard evaporation, but total recovery levels were still low. 3 skin samples came from each of 3 pigs. Results were given as penetration of unchanged DEM and penetration of monoethyl malonate and malonic acid. I have combined these together to give the overall penetration.

REFERENCE

Distribution And Fate Of Diethyl Malonate And Diisopropyl Flurophosphate On Pig Skin In Vitro (1988) J. Pharm. Sci., 77 :850-854