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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 99

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dimethylethylamine

0.67

0.1

Water

Guinea pig

Full Thickness Skin

Not Given

Flow-through

Hanks Balanced Salt Solution

Not Given

Gas Chromatography

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										5		0.001	6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.001

NOTES

mean bw 640g. The curve shape did not allow lag time calculation. 6 flow-through cells were run.They tested for both DMEA and it's metabolite DMEAO in the receptor fluid. No DMEAO was found

REFERENCE

Skin Absorption Of The Industrial Catalyst Dimethylethylamine In Vitro In Guinea Pig And Human Skin, And Of Gaseous Dimethylamine In Human Volunteers (1997) J. Pharm. Biomed. Anal., 70 :309-313