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In Vitro Study No: 991

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Paraquat 0.1 WaterMarmosetFull Thickness SkinBack2.54StaticSaline30Water Bath662Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            6.4E-0517 

NOTES

An integriy test was done with tritiated water, skin membranes were then allowed to hydrate naturally after a small quantity of saline was placed in the receiver chamber and left overnight. Graphs were drawn of the amount penetrated vs time and ss-flux was calculated form the linear portion of the graph. Kp was calculated by dividing the ss-flux by the applied concentration. The concentration of paraquat applied was 1mg/ml + 6.26 mcrCi/m.l The specific activities of the neat compounds were not given so I was unable to work out the actual concentration applied in mg/ml

REFERENCE

The Influence Of Skin Structure On Permeability: An Intersite And Interspecies Comparison With Hydrophilic Penetrants (1991) J. Investig. Dermatol., 96 :921-925