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In Vivo Study No: 10

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Hydrocortisone0.020.50.004AcetoneHumanForearm2.5224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
168    0.000191.9  0.000161.61.21.6
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     10  

NOTES

Total absorption was calculated by measuring the amount eliminated in the urine and corrected with comparison to the fraction of an intravenous dose eliminated in the urine. The amount left in the Stratum Corneum was measured by tape stripping.

REFERENCE

Percutaneous Absorption Of Hydrocortisone And Testosterone On The Vulva And Forearm: Effect Of The Menopause And Site (1996) Br. J. Dermatol., 134 :229-233