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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 10

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Hydrocortisone	0.02	0.5	0.004	Acetone	Human	Forearm	2.5	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168					0.00019	1.9			0.00016	1.6	1.2	1.6

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

168

0.00019

1.9

0.00016

1.6

1.2

1.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					10

NOTES

Total absorption was calculated by measuring the amount eliminated in the urine and corrected with comparison to the fraction of an intravenous dose eliminated in the urine. The amount left in the Stratum Corneum was measured by tape stripping.

REFERENCE

Percutaneous Absorption Of Hydrocortisone And Testosterone On The Vulva And Forearm: Effect Of The Menopause And Site (1996) Br. J. Dermatol., 134 :229-233