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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 100

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Caffeine	0.02	9.71	0.1942	ethyleneglycol/H2O/tritonx100 (90/10)/[H2O] (50/50)	Human	Upper Arm	1	2	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24									0.00235	1.209	0.184	1.209

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00235

1.209

0.184

1.209

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					7

NOTES

Caucasian Males were used. The % Absorption after 4 days (B) was extrapolated from the amount excreted in the urine after 24h (A) using the equation (B) = (A)/0.5 (from data in literature on the kinetics of urinary excretion of these substances)

REFERENCE

An Original Predictive Method For In Vivo Percutaneous Absorption Studies (1987) J. Soc. Cosmet. Chem., 38 :397-417