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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 104

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Acetylsalicylic Acid0.029.030.18016ethyleneglycol/H2O/tritonx100 (90/10)/[H2O] (50/50)HumanUpper Arm120.5Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
24        0.003061.70.0371.7
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     7  

NOTES

Caucasian Males were used. The % Absorption after 4 days (B) was extrapolated from the amount excreted in the urine after 24h (A) using the equation (B) = (A)/0.31(from data in literature on the kinetics of urinary excretion of these substances)

REFERENCE

An Original Predictive Method For In Vivo Percutaneous Absorption Studies (1987) J. Soc. Cosmet. Chem., 38 :397-417