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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 110

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Benzoic Acid	0.02	1.221	0.02442	Propylenglycol-triton X-100 (90:10)	Rat	Dorsal	1	2	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
96							4E-06	0.015	0.00014	0.575	0.06	0.575

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

4E-06

0.015

0.00014

0.575

0.06

0.575

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					12

NOTES

% Absorption was determined by adding the % in urine + faeces + epidermis + dermis + whole body after sacrifice. The animals were anaethetised before application of the chemical.

REFERENCE

The Measurement Of The Stratum Corneum Reservoir: A Simple Method To Predict The Influence Of Vehicles On In Vivo Percutaneous Absorption (1986) Br. J. Dermatol., 115 :233-238