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In Vivo Study No: 123

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
DimethylethylamineInfinite0.00027 AirHumanForearm872.314Gas Chromatography

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
         0.04467   
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
 0.01330.00047  30.0495 

NOTES

As the dose was infinite, evaporation did not affect the study, so it is recorded as an occluded study. The subjects were 2 women and one man all Caucasian. The mean concentration of DMEA was 270mg/m3 and the chamber had a volume of 0.5m3. The 3 subjects had slightly different exposure areas, due to their differing sizes of forearms. Subject A - 891cm2, B - 891cm2, C - 835cm2. the mean has been used in the database.The mean uptake was 44.75microg. I calculated the average flux and standard deviation from the raw flux results. They calculated Kp from the amount absorbed/surface ares exposed x exposure concentration x exposure time, and then calculated Jss from that so there is no way of knowing whether steady sta conditions were actually reached.

REFERENCE

Skin Absorption Of The Industrial Catalyst Dimethylethylamine In Vitro In Guinea Pig And Human Skin, And Of Gaseous Dimethylamine In Human Volunteers (1997) J. Pharm. Biomed. Anal., 70 :309-313