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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 14

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Testosterone	Not Given		0.0133	Acetone	Monkey	Cheek	6	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120									0.00766	9.6	0.35	9.6

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

0.00766

9.6

0.35

9.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Female monkeys were used. 0.0798mg of Testosterone was added in total. The data was corrected for incomplete urinary recovery. The results were given as +/- SEM I have converted these to SD. The monkeys were not anaethetised. They were kept in metabolism chairs with their arms secured for 24h to prevent them wiping the chemical off the skin.

REFERENCE

Variations In Percutaneous Absorption Of Testosterone In The Rhesus Monkey Due To Anatomical Site Of Application And Frequency Of Application (1980) Toxicol. Lett., 267 :229-235