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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 154

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Beta-Estradiol	6	31.1	149.28	Ethanol	Human skin grafted rat	Abdomen	0.8	1	4	Radiolabelled (3H)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
	53.9	7.79			3	0.0019

NOTES

With infinite dosed experiments, evaporation is not a factor so it is as though the cell was occluded. They used a STeCC donor cell to apply the chemical, this had a total volume of 6ml and kept the donor concentration constant. Dermatomed (0.5mm) Human skin from an adominoplasty was used and left for 5 weeks after grafting. Rats were anaethetised during the experiment. Results were given +/- SE. I have converted these to SD for the database.

REFERENCE

Mechanism of Ethanol-Enhanced Estradiol Permeation Across Human Skin In Vivo (1990) Pharmaceut. Res., 7 :170-175