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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 16

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Testosterone	0.5	19.4		Methylcellosolve	Monkey	Vagina		2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
240									6.1207	63.1	3.68	63.1

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

240

6.1207

63.1

3.68

63.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					2

NOTES

The monkeys were anaethetised with 1mg/kg phencyclidine hydrochloride. A catheter was placed in the bladder. The dose was pipetted into the vagina and a latex plug with gauze padding was inserted this was removed after 24h and the area was washed. The results were given as +/- SEM I have converted these to SD

REFERENCE

Variations In Percutaneous Absorption Of Testosterone In The Rhesus Monkey Due To Anatomical Site Of Application And Frequency Of Application (1980) Toxicol. Lett., 267 :229-235