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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 163

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Hydrocortisone	Not Given		0.004	Acetone	Human	Forearm	13	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120									0.00097	1.87	1.59	1.87

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

0.00097

1.87

1.59

1.87

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					15

NOTES

The % absorption was calculated by measuring the amount excreted in the urine and comparing this with the amount excreted after a parenteral dose. The skin area was enclosed in a ring of petrolatum. The acetone was evaporated in less than 15 seconds from the application.

REFERENCE

Percutaneous Penetration Of Steroids In Man (1969) J. Investig. Dermatol., 52 :89-94