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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 168

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Fluocinolone Acetonide	Not Given		0.004	Acetone	Human	Forearm	500	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120									0.0268	1.34	1.05	1.34

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

0.0268

1.34

1.05

1.34

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					9

NOTES

The % absorption was calculated by measuring the amount excreted in the urine and comparing this with the amount excreted after a parenteral dose. The skin area was enclosed in a ring of petrolatum. The acetone was evaporated in less than 15 seconds from the application. An area of 500cm3 had to be used due to the low specific activity

REFERENCE

Percutaneous Penetration Of Steroids In Man (1969) J. Investig. Dermatol., 52 :89-94