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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 20

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Testosterone	0.05	0.14	0.004	Acetone	Guinea pig	Back	1.75	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120			16	1.6					0.0035	50.7	12.3	50.7

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

1.6

0.0035

50.7

12.3

50.7

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					5

NOTES

Corrected for incomplete excretion using data obtained from parenteral administration. Excretion Correction Factor = 2.13 These high values may be false due to low recovery in urine after parenteral administration. (46.8%). The alternative method of adding urine and faeces may be better (see next study)

REFERENCE

The Guinea-Pig: An Animal Model For Human Skin Absorption Of Hydrocortisone, Testosterone And Benzoic Acid? (1980) Br. J. Dermatol., 102 :447-453