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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 200

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Cortisone	Not Given			Petrolatum	Rat	Back	2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120										19.6	2.6	19.6

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

19.6

2.6

19.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Amount absorbed was determined by amount excreted in urine corrected with amount excreted from a intraperitoneal injection in other animals. The skin was protected by a nylon ring attached to the skin with a cyanoacrylate adhesive. The removal of this device was prevented by tying rubber tubing immediately behind the front legs and in front of the back legs. Further experimental detail - Bronaugh et al. Toxicol. Appl. Pharmacol. (1982) 62 474. Correction Factor was 21% The results were given as +/- SEM I have changed this to SD for the database.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies IV: The Flow-Through Diffusion Cell (1984) J. Pharm. Sci., 74 :64-67