EDETOX logo

IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 220

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Ketoprofen	See Notes		0.587	0.05% Nonivamide, 30% Ethanol & Formax plus gel	Rat	Abdomen	2.3	1	10	HPLC

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3		0.03

NOTES

An occlusive dressing was used to occlude the exposure site. Male Wistar Rats were anaethetized. Gel was made from hydroxypropy cellulose dissolved in water and propylene glycol. Ketoprofen was dissolved in ethanol andmixed with the gel. 45mg of gel containing 3% Ketoprofen was added in total

REFERENCE

Evaluation Of Percutaneous Absorption And Skin Irritation Of Ketoprofen Through Rat Skin: In Vitro And In Vivo Study (2001) Int. J. Pharm., 222 :225-235