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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 232

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Benzoic Acid	See Notes		0.0005	Petrolatum	Human	Abdomen		2	24	Radilabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
										60.6	21.4	60.6

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

60.6

21.4

60.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Non-Occlusive guards were used to prevent loss of the dose. 5mg/cm2 of Petrolatum was added. The dose area was between 10 and 20 cm2. The % absorption was calculated by comparing the amount excreted in the urine with the amount excreted from a parenteral dose and then correcting for incomplete excretion. It was given +/- SEM. I have converted this to SD for this database.

REFERENCE

Vehicle Effects On Percutaneous Absorption: In Vivo And In Vitro Comparison With Human Skin (1986) Br. J. Dermatol., 115 :1-11