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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 235

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
CaffeineSee Notes 0.05Water GelHumanAbdomen 224Radilabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
          414
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

The Gel was made using a 1.5% solution of Carbopol. To form the gel the solution was neutralised with 10% NaOH. 400mg/cm2 of water gel was added. Non-Occlusive guards were used to prevent loss of the dose. The dose area was between 10 and 20 cm2. The % absorption was calculated by comparing the amount excreted in the urine with the amount excreted from a parenteral dose and then correcting for incomplete excretion. It was given +/- SEM. I have converted this to SD for this database.

REFERENCE

Vehicle Effects On Percutaneous Absorption: In Vivo And In Vitro Comparison With Human Skin (1986) Br. J. Dermatol., 115 :1-11