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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 24

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Caffeine	0.01	0.508	0.004	Ethanol	Mouse	Anterior End	1.27	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
	92	6	0.00112	22			3E-06	0.05	0.00356	70.1		70.1

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00112

3E-06

0.05

0.00356

70.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Athymic, Hairless Mice were used. Radioactivity was measured until the amount present in the urine was equal to background levels. The % Absorption was derived by adding the % of the topically applied radioactive dose in urine, faeces & carcass - application site Radioactive dose 0.05microCi.

REFERENCE

Percutaneous Penetration In The Hairless Dog, Weanling Pig And Grafted Athymic Nude Mouse (1984) Br. J. Dermatol., 111 :123-135