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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 254

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Fluazifop-butyl	4	0.5	0.0025	Commercial Emulsifyable Concentrate	Human	Back	800	2	8	Gas chromatography/Mass Spectroscopy

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168									0.159	7.95	1.55	7.95

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

168

0.159

7.95

1.55

7.95

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					6

NOTES

The site was washed with distilled water after 8 hours and the volunteers showered after 24h. Both plasma and urine samples were analysed. They calculated the absorbed dose at discrete timepoints by adding the cumulative urinary excretion to the bosy burden. They found that absorption did not occur to any significant extent after 24 hours which is when the volunteers showered.

REFERENCE

The Predictive Accuracy Of In Vitro Measurements For The Dermal Absorption Of A Lipophilic Penetrant (Fluazifop-Butyl) Through Rat And Human Skin (1994) Fundam. Appl. Toxicol., 23 :230-236