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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 261

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Coumarin	0.2	0.9	0.02	70% aq. Ethanol	Rat	Back	9	1	6	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120										69.3	5.05	69.3

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

69.3

5.05

69.3

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					2

NOTES

The application site was occluded with Aluminium foil. Male Lister-Hooded Rats were used. More detail can be obtained from Mayo B, (1996) Study No: RIF 30/943257 (Research Institute for Fragrance Amterials Inc., USA. Skin absorption was assessed indirectly by combining the radioactivity recovers in the urine and faeces.

REFERENCE

Comparative In Vitro Skin Absorption And Metabolism Of Coumarin (1,2-Benzopyrone) In Human, Rat and Mouse (1997) Toxicol. Appl. Pharmacol., 145 :34-42